Effect of Marin Extract on Blood in Rats

• Farida Hussain Bouhteira

  Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Al-Bayda, Libya

  Author

• Nourah Ibrahim Al-Zayel

  Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Al-Bayda, Libya

  Author

• Abdel Salam Musa Bawalhaj

  Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Al-Bayda, Libya

  Author

• Saad Gharbawi

  Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Al-Bayda, Libya

  Author

The aim of this study was to test the effect of the ethanolic extract of Myrtus leaves on the blood picture in White Rats. The animals were given a dose of 2 g/kg orally. A total of 28 rats were divided into 2 groups; 14 rats in each group. Each group included 7 animals as a control. The first group was given the dose daily for 7 days. The second group was treated for 14 days. 

Some of the animals treated with the extract showed bleeding through mouth and nose. They were lethargic, tired and lost apetite. Furthermore, the weight of these animals was significantly lower than those in the controls.

With  regard to blood picture , the treatment for one week caused significant reduction in the number of red blood corpuscles, packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin concentration and number of platelets, while there were a significant increase in the white blood cells and lymphocytes. However, treating the animals with the extract for two weeks caused a significant reduction in the number of red blood corpuscles, packed cell volume , heamoglobin estimation and number of blood platelets, while there was an increase in mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, white blood cells and the number of lymphocytes.

العمامي، عطية علي أحمد (1999) تحليل بعض مكونات نوى التمر وتقييمه حيوياً كمشروب ساخن (قنوي). أطروحة ماجستير. قسم علوم الأغذية. كلية الزراعة. جامعة الفاتح.

القاضي، عبدالله عبدالحكيم (1954) استعمالات بعض النباتات في الطب الشعبي الليبي. الجزء الثالث، دار الحكمة للطباعة والنشر والتوزيع، ليبيا، 107- 109.

الكبيسي، خالد (2002) الكيمياء الحيوية، العلوم الطبية المساعدة. الطبعة الأولى، دار وائل للنشر والتوزيع، عمان، الأردن.

شيفيل، نورمان (1982) أمراض الخلية. ترجمة: غياث صالح محمود 1987، الطبعة الأولى، مطبوعات جامعة الموصل، العراق.

علي، رابحة عيسى (2007) تأثير التغذية بالحلبة في بطانة الرحم وبعض المعايير الفسيولوجية لإناث الجرذان. أطروحة ماجستير، قسم علم الحيوان، جامعة عمر المختار، ليبيا.

المرغني، فتحية عتيق (2005) دراسة بعض التأثيرات البيولوجية لمستخلص نبات المرسين Myrtus communis L Myrtaceae، على كروموسومات نقي العظم للفئران والقمم النامية لجذور نبات البصل. أطروحة ماجستير، قسم علم الحيوان، جامعة عمر المختار، ليبيا.

الهواري، محمد فتحي وعزالدين، فؤاد وهبي (1981) الكيمياء السريرية. الجزء النظري الأول، منشورات مكتبة المعارف الحديثة، المعهد الطبي الفني، بغداد.

زايد، عبدالله عبدالرحمن ومبارك، عبدالرحمن خوجلي (1995) علم وظائف الأعضاء العام. الطبعة الأولى، منشورات جامعة عمر المختار، البيضاء – ليبيا.

Arun, N. and Nalini, N. (2002) Effecincy of Turmeric on blood sugar and polyol pathway in diabetic albino rats. Plant Foods Hum Nutr., 57, (1), 41-52.

Baker, F. J. and Silverton, R. E. (1985) Introduction to medical laboratory technology. Butterworths, London., 6th edition, 303-347.

Bastianetto, S. (2000) Biological activities of the components of Ginkgo biloba. J. Neurochemistry., 74, 2268-2277.

Bin-Hafeez, B., Haque, R., Parvez, S., Pandey, S., Sayeed, I. and Raisuddin, S. (2003) Immunomodulatory effects of fenugreek (Trigonella foenum-graecum L) extract in mice. Int. Immunopharmacol., 32, 257-265.

Bracco, D., Ferrarra, J. M., Arnaud, M. J., Jequier, E. and Schutz, Y. (1995) Effects of caffeine on energy metabolism, heart rate and methylxanthine metabolism in lean and obese women. Am. J. Physiol., 269, (4part1), E671-E678.

Coles, D. V. M. and Embert, H. (1986) Veterinary clinical pathology. W.B. Saunders Company, 4th edition.

Deng, Z. Y. and Tao, B. Y. (1998) Effect of green tea and black tea on blood glucose, triglycerides and antioxidants in aged rats. J. Agricult Food Chem., 46, 3875-3878.

El-Fellah, M. S., Akhter, M. H. and Khan, M. T. (1984) Anti-hyperglycaemic effect of an extract of Myrtus communis in streptozotocin-induced diabetes in mice. J. Ethnopharmacol., 11, (3), 275-281.

Feibt, C., Franke, L., Appendino, G. and Werz, O. (2005) Identification of molecular targets of the oligomeric nonprenylated acylphloroglucinols from Myrtus communis and their implication as anti-inflammatory compounds. J. Phamacology and Experimental Therapeutics., 315, 389-396.

Gartner, R. J. W. and Hurwood, I. S. (1976) The tannin and oxalic acid content of Acacia aneura (mulga) and their possible effects on sulphur and calcium availability. Australian Veterinary Journal., 52, 194-195.

Griffiths, D. (1982) The phytate content and iron binding capacity of various field bean (Vicia faba) preparations and extracts. J. Science and Food in Agriculture., 33, 847-851.

Han, Y., Nishibes-Noguchi, Y. and Jin, Z. (2001) Flavonol glycosides from the stems of Trigonella foenum-graecum. Phytochemistry., 58, (4), 577-580.

Hannan, J. M., Rokeya, B., Farugue, O., Nahar., Mosihuzzaman, M., Aza-Khan, A. K. and Ali, L. (2003) Effect of soluble dietary fiber fraction of Trigonella foenum-graecum on glycemic, insulinemic, lipidemic and platelet aggregation status of type-2 diabetic model rats. J. Ethnopharmacol., 88, 73-77.

Jain, N. C. (1986) Schalm's veterinary hematology. Lea & Febiger, Philadelphia, 4th edition, 35-44.

Mbatha, K. R., Downs, C. T. and Nsahlai, I. V. (2005) The effects of graded levels of dietary tannin on the epithelial tissue of the gastro-intestinal tract and liver and kidney masses of Boer goats. Med. J., 74, (3), 3209.

Mehansho, H., Hagerman, A., Clements, S., Butler, L., Rogler, J. C. and Carlson, D. M. (1983) Modulation of proline-rich protein biosynthesis in rat parotid gland by sorghums with high tannin levels. Proc. Nat. Acad. Sci., USA, 80, 3948.

Ong, K. C., Khoo, H. E. and Das, N. P. (1995) Tannic acid inhibits insulin-stimulated lipogenesis in rat adipose tissue and insulin receptor function in vitro. Experientia., 51, (6), 577-584.

Ott, L. (1984) An introduction to statistical methods and Data Analysis. Duxburg Press, Boston, USA, 2nd edition.

Petit, P., Sauvaire, Y., Ponsin, G., Manteghetti, M., Fave, A. and Ribes, G. (1993) Effect of fenugreek seed extract on feeding behaviour in the rat : metabolic-endocrine correlates. Pharmacol. Biochem. Benav., 45, (2), 369-374.

Sato, T., Onse, Y., Nagase, H. and Kito, H. (1990) Mechanism of antimutagenicity of aquatic plant extracts against benzo(a) pyrene in the Samonella assay. Mut. Res., 241, 283-290.

Schalm, O. W., Jain, N. C. and Carroll, E. J. (1975) Veterinary hematology. Lea & Febiger, Philadelphia, 3rd edition, 239-241.

Yousef, M. I., El-Demerdash, F. M., Kamel, K. I. and Al-Salhen, K. S. (2003) Changes in some hematological and biochemical indices of rabbits induced by Isoflavones and cypermethrin. Toxicol., 189, 223-234.

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