The Effect of Salinity on Wheat Genotypes during Germination Stage

• Amal Ehtaiwesh

  Department of Plant, Faculty of science, University of Zawia, Zawia, Libya

  Author

Salinity is a major abiotic stress that adversely affects wheat production in many regions of the world. Salinity stress limits wheat growth, development, and yield. Identification of salinity tolerant genotypes is critical for yield improvement. Therefore, a series of control environment experiments were carried out to evaluate the response of two spring wheat and two winter wheat cultivars (Triticum aestivum L.) to different levels of salinity. The experiments were designed in a randomized complete block design (RCBD) with five replications. Twenty seeds of each genotype were placed on pre-moistened filter paper in Petri dishes and placed in an incubator at 20 °C. The seeds were subjected to 4 levels of salinity 0, 50, 100, and 150 mM NaCl. Seedlings were harvested after 8 days, and data on final germination percentage, rate of germination, mean daily germination, shoot and root length, and seedling fresh and dry weight were recorded. The results indicated that winter and spring wheat genotypes differed significantly for germination percentages, rate of germination, mean daily germination, shoot and root lengths, and seedling fresh and dry weight. The results showed that salinity did not affect final germination percentage until salinity level reached to 100 mM NaCl; whereas seeds subjected to 100 and 150 mM NaCl retarded germination by 1 and 2 days of spring wheat, and 2-3 days of winter wheat respectively, as compared with 0 and 50 mM NaCl treatment. The data also showed that increasing salinity level significantly decreased shoot and root length, however, the study found that salinity affected root growth more severely than shoot growth of seedlings. Significantly, root length and dry weight of root ranked genotypes in the same order as their salt-tolerance. Therefore, the study concluded that the measurements of root growth would be effective criteria for screening wheat genotypes for salt tolerance at seedling stages.

Abdoli, M., Saeidi, M., Azhand, M., Jalali-Honarmand, S., Esfandiari, E., and She-kari, F. (2013). The Effects of different levels of salinity and indole-3-acetic acid (IAA) on early growth and germination of wheat seedling. Journal of Stress Physiology and Biochemistry 4: 329-338.

Acevedo, E., Silva, P., and Silva, H. (2002). Wheat growth and physiology. In: Cur-tis, B. C., S. Rajaram and H. G. Mac-pherson (Eds.) Bread Wheat Improve-ment and Production, FAO Plant Pro-duction and Protection Series, No. 30. Food and Agriculture Organization of the United Nations, Rome, Italy.

Adjel, F., Kadi1, A. Z., Bouzerzour, H., and Benmahammed, A. (2013). Salt stress effects on seed germination and seedling growth of barley (Hordeum vulgare L.) genotypes. Journal of Agriculture and Sustainability 2: 223-237.

Akbarimoghaddam, H., Galavi, M., Ghanbari, A. and Panjehkeh, N. (2011). Salinity Effects on Seed Germination and Seed-ling Growth of Bread Wheat Cultivars. Trakia Journal of Sciences. 9: 43-50.

Andersen, P. P., Lorch, R. P., and Rosegrant, M. W. (1999). World food prospects: critical issues for the early twenty-first century. Food policy report. International food policy research institute Washing-ton, D.C.

Barnes, J.N., and Shields, D. A. (1998). The growth in U.S. wheat food demand. Economic Research Service/USDA. Wheat Yearbook/WHS /March 1998. Pp 21.

Carte, B. (2002). The importance of wheat quali-ty. Agricultural Horizons. Washington State University. US. Department of Ag-riculture, and Eastern Washington Coun-ties. Cooperative. pp 2-12.

Catalan, L., Balzarini, M., Taleisnik, E., Sereno, R., and Karlin, U. (1994). Effects of sa-linity on germination and seedling growth of Prosopis flexuosa (D.C.). Forest Ecology and Management 63: 347-357.

Djanaguiraman, M., and P. V.V. Prasad. (2013). Effects of salinity on-ion transport, water relations and oxidative damage. In: Ecophysiology and respons-es of plants under salt stress. (Eds.) Ahmad, P., Azooz, M.M., Prasad, M.N.V. Springer New York. pp 89-114.

Dubcovsky, J., and Dvorak, J. (2007). Genome plasticity a key factor in the success of polyploid wheat under domestication. Science 316: 1862-1866.

FAO. (2008). FAO Land and Plant Nutrition Management Service. http://www.fao.org/ agl/agll/spush.

FAO. (2013). FAO statistical yearbook. Availa-ble at http://www.fao.org/ docrep/018/i3107e/i3107e.pdf. Food and Agriculture Organization of the United Nations Rome, Italy.

Francois, L.E., Maas, E. V., Donovan, T. J., and Youngs, V. L. (1986). Effect of salinity on grain yield and quality, vegetative growth, and germination of semi-dwarf and durum wheat. Agronomy Journal 78: 1053-1058.

Gairola, K.C., Nautiyal, A. R., and Dwivedi, A. K. (2011). Effect of temperatures and germination media on seed germination of Jatropha Curcas Linn. Advances in Bioresearch 2: 66-71.

Hussain, S., Khaliq, A., Matloob, A., Wahid, M. A., and Afzal, I. (2013). Germination and growth response of three wheat cul-tivars to NaCl salinity. Soil and Envi-ronment 1: 36-43.

Islam, R., A. Mukherjee, and M. Hossain. 2012. Effect of osmopriming on rice seed ger-mination and seedling growth. J. Bang-ladesh Agric. Univ. 10(1): 15–20.

Jamil A., Riaz S., Ashraf M.,and Foolad M.R. (2011). Gene expression profiling of plants under salt stress. Crit. Rev. Plant Sci. 30(5):435–458.

Karim, M.A., Utsunomiya, N., and Shigenaga, S. (1992). Effect of Sodium on germina-tion and growth of hexaploid triticale at early seedling stage. Japanese Journal of Crop Science 61: 279-284.

Kayani, S.A., Naqvi, H.H., and Ting, I.P. (1990). Salinity effects on germination and mobilization of reserves in Jojoba seed. Crop Science 3: 704-708.

Kazemi, K., and Eskandari, H., (2011). Effects of salt stress on germination and early seedling growth of rice (Oryza sativa) cultivars in Iran. African Journal of Bio-technology 77: 17789-17792.

Khayatnezhad, M, and R. Gholamin. 2010. Study of Nacl salinity effect on wheat (Triticum aestivum L.) cultivars at ger-mination stage. Journal of Agriculture and Environmental Sciences. 9 (2): 128-132.

Kumar, M., Singh, M. P., and Kumar, S. (2012). Effect of salinity on germination, growth, yield and yield attributes of wheat. International Journal of Scientific and Technology Research. 6: 19-23.

Muhammad, Z. and Hussain, F. (2012). Effect of NaCl salinity on the germination and seedling growth of seven wheat geno-types. Pakistan Journal of Botany 6: 1845-1850.

Munns, R., James, R. A., and Lauchli, A. (2006). Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany. 57: 1025-1043.

Muthusamy, S. K, Dalal, M., Chinnusamy, V.,and Bansal, K. C. (2017). Genome-wide identification and analysis of biotic and abiotic stress regulation of small heat shock protein (HSP20) family genes in bread wheat. Journal of Plant Physiolo-gy 211: 100-113.

Nasr, S.M.H., Parsakhoo, A., Skandari, S., Gojani, H.J. and Koohi, S. K. S. (2012). Investigation of salinity solerance in Do-donaea viscosa L. Journal of applied bi-ological sciences. 6: 31-36.

Nasri, N., Kaddour, R., Rabhi, M., Plassard, C., and Lachaâl, M. (2011). Effect of salini-ty on germination, phytase activity and phytate content in lettuce seedling. Acta Physiologiae Plantarum, 33: 935-942.

Nedjimi, B. (2014). Effects of salinity on growth, membrane permeability and root hydraulic conductivity in three saltbush species. Biochem. Syst. Ecol. 52, 4–13.

Oleson, B. T. (1994). World wheat production, utilization and trade. In: Bushuk, W. and Rasper, V.F (Eds.). Wheat: Production, Properties and Quality. Bla-ckie/Chapman and Hall, London, pp. 1-11.

Plaut, Z., Edelstein, M., and Ben-Hur, M. (2013). Overcoming salinity barriers to crop production using traditional meth-ods. Critical Reviews in Plant Sciences 32: 250-291.

Qadir, M., Tubeileh, A., Akhtar, J., Larbi, A., Minhas, P. S., and Khan, M. A. (2008). Productivity enhancement of salt affected environments through crop diver-sification. Land Degradation and Devel-opment 19: 429-453.

Rahman, M., Soomro, U. A., Zahoor-ul-Haq, M., and Gul, S. (2008). Effects of NaCl salinity on wheat (Triticum aestivum L.) cultivars. World Journal of Agricultural Sciences 3: 398-403.

Rengasamy, P. (2006). World salinization with emphasis on Australia. Journal of Exper-imental Botany 5: 1017-1023.

Rubio-Casal, A.E., Castillo, J. M., Luque, C. J., and Figueroa, M. E. (2003). Influence of salinity on germination and seeds viabil-ity of two primary colonizers of Mediter-ranean salt pans. Journal of Arid Envi-ronments 53: 145-154.

Setter, T. L.; Waters, I.; Stefanova, K.; Munns, R. (2016) Salt tolerance, date of flower-ing and rain affect the productivity of wheat and barley on rainfed saline land. Field Crops Res. 194, 31–42.

Shahbaz M.,and Ashraf M. (2013). Improving salinity tolerance in cereals. Crit. Rev. Plant Sci. 32:237-249.

Shrivastava, P., and Kumar, B. (2015). Soil sa-linity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences. 22: 123-131.

Tanji, K.K. (1990). Nature and extent of agricul-tural salinity. In: Tanji KK (Ed.). Agri-cultural Salinity Assessment and Man-agement. Manuals and Reports on Engi-neering Practices No. 71. American So-ciety of Civil Engineers: New York; 1-17.

Tsegay, B. A., and Gebreslassie, B. (2014). The effect of salinity (NaCl) on germination and early seedling growth of Lathyrus sativus and Pisum sativum var. abyssini-cum. African Journal of Plant Science 5: 225-231.

Tuteja, N. (2007). Mechanism of high salinity tolerance in plants. Methods in Enzy-mology 428: 419–438.

Wang, Y. R., Kang, S. Z., Li, F. S., Zhang, L. and Zhanf, J. H. (2007). Saline water ir-rigation through a crop-water-salinity production function and a soil water- sa-linity dynamic model. Pedosphere 17: 303-317.

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