Quinine effects on normal spontaneous activity and on acetyl choline–induced contractures of rabbit ileal smooth muscle

• Khalid. H. M. Saad

  Biology Dept. Omar El-Mukhtar University

  Author

The aim of this study is to know the effect of quinine on rhythmic contractions of rabbit ileal smooth muscle and on the contractures induced by acetyl chorine. In low concentrations (0.05–0.2 mM) quinine caused gradual decline of spontaneous activity and filially abolished it completely. Moderate and high concentration (0.5–5 mM) of quinine brought about rapid relaxation, but did not effect the basic tension. The moderate concentration of quinine relaxed the muscle, which was induced to contracture by Acetyl choline. When the muscles bathed in Ca–Free solution lost their normal spontaneous contraction and normal calcium returned to the media in the presence of moderate concentrations of quinine failed to return to normal activity. These results may suggest that quinine may exert its relaxatory effect by blocking the inward calcium movement from the extra cellular medium which believed to be the trigger for smooth muscle normal contractions since this smooth muscle lack well developed sarcoplasmic reticulum

Alexander–PB; Cheung–DW (1994), Ca2+ mobilzation by caffeine in single smooth muscle cells of the rat tail artery.

Barratt, L. and H. Huddarat (1979), Spontaneous activity and related Ca2+ movement of fish intestinal smooth muscle. Gen. Pharrnac. 10 21–30.

Barratt, L. (1989), Spontaiieous activity aiid related calcium movements of fish inestinal smooth. Ph. D Thesis.

Batra, S. (1974), The effect of drugs Ofl calcium uptake atid calcium release by mitochondria and sarcoplasmic reticulum of frog skeletal muscle. Blochem. Pliarmac. 23 89–101.

Batra, S. (1976), Mitochondria calcium release as a mechanism for quinldlne contracture in skeletal muscle biochem. Pharmac. 25. 263 1–2633.

Batra, S. (1977), The importance of calcium binding by subcellular components of smooth muscle. In “Excitation–contraction coupling in smooth muscle”. pp. 225–232.

Ebashi, S. and M. Endo (1968), Calcium ion and muscle contraction. Prog. Biophys. Molec. Biol. 19, 125–1 83.

Huddart, H. (1972) Factors modifying caffeine and quinine contractures and the recovery of contractility in crab skeletal muscle. comp. Biochem. Physiol. 43 A, 369–379.

Huddart, H. and Hunt (1976), Visceral muscle – its structure and function. Biackie, Glasgow.

Huddart, H. and A. J. Sysoii. (1975), The effect of caffeine on calcium efflux and calcium transolocation in skeletal and visceral muscls. J. Exp. Biol. 63, 13 1–142.

Huddart, H. and M. W est (1975), Quinine stimulation of Ca2+ effux from arthropod skeletal muscle in relation to quinine effects on calcium translocation and binding. Expermentia, 3, 665–667.

Huddart, H. and N.R. Price (1976), Calcium movements during excitation–contraction coupling in muscle cells. Comp. Blochern. Physiol 541/375–386.

Huddart, H. and K. H. M. Saad (1977). Quinine and Lanthanum effect on contractility and calcium movements of rat ileal smooth muscle. gen Pharmac. 8, 34 1–347.

Huxley, H. E. (1969). The mechanism of muscle contraction. Science, 164 (1966–1969).

Isaacson. A. and A. Sandow (1967). Quinine and caffeine effects on 45Ca movements in frog sartoious muscle. J. Gen Piiysiol. 50, 2109– 2128.

Paul, D. J. (1972). Cellular calcium pool for contraction of rabbit ileal muscle, can. J. Physiology Pharmacol. 51, 260–270.

Pickkers–P; Hughes–AD (1995). Relaxation and decrease in [Ca2+] byhydrochlorothiazide in guinea–pig 3 1 of 64 isolated mesenteric arteries.

Saad, K. H. M. (1980). Calcium regulation during excitaion–contraction coupling of mammalian smooth muscle. Ph. D. Thesis University of Lancaster.

Sanger, I. W. and R. B. Hill (1973). The contractile apparatus of radula protractor muscle of busycon canaliculatum. Proc. Maiac. Soc. London 40, 335–341.

Saiidow, W. A. (1970). Skeletal muscle. A. Rev. Physiol. 32, 87–138.

Syson, A. J. (1974). Studies on the excition–contraction coupling mechanism of mammalian smooth muscle. Ph. 5. Thesis, University of mammalian Lancaster.

Syson, A. J. and H. Huddart(1976). The effect of caffeine on excitation-contraction coupling in skeletal and smooth muscle. J. Exp. Biol. 64, 789–798.

Weber, A. R. Herz and I. Reiss (1963). On the mechanism of the relaxatory effect of fragmented sarcoplasmic reticurn. J. Gen. Physiol. 45, 679–702.

Weiss, G. B. (1974). Cellular pharmacology of Lanthanum. Ann Rev. Pharmac. 14, 343–354.

Weiss, G. B. and F. R. Goodman. J. pharmac. Exp. Ther. 198, 366–374.

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