ISSR Analysis to Detect Genetic Variation Among Some Lentil Genotypes in Syria

• Rehab Al-Mousa

  Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria

  Author

• Alaa Alshaal

  Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria

  Author

• Shahinaz Abbas

  Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria

  Author

• Khouzama Kountar

  Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria

  Author

Lentil (Lens culinaris Medik) plays a significant role in human and animal nutrition. It is important to generate baseline information on genetic diversity of local and introduced lentil in Syria. Therefore, this study aimed to assess diversity and relationship among two local varieties and 35 introduced lentil lines from ICARDA using 20 Inter Simple Sequence Repeats (ISSR) primers.18 primers were able to amplify DNA bands with a total of 184 bands. Primers ISSR5, ISSR11, ISSR13, ISSR15 and ISSR16 presented 100% polymorphism percentage. The mean value of polymorphism percentage was 87.31%. ISSR primers could identify 19 unique bands in all genotypes, 12 of them were positive bands (present) and 7 were negative bands (absent). Primer ISSR11 was able to detect 3 positive unique bands in genotype Ln20. The UPGMA (unweighted pair group method with arithmetical averages) clustering based on ISSR data grouped 37 lentil genotypes into 6 clusters. The lowest genetic distance (0.20) was observed between (Ln15 and Ln16, Ln21 and Ln22, Ln27 and Ln28) which proves that they are closely related. While Ln24 presented the highest genetic distance (0.49) with both Ln18 and Idleb3 which refers to their genetic divergence.

Arumuganathan, K., & Earle, E. (1991). Nuclear DNA content of some important plant species. Plant molecular biology reporter, 9, 208-218.

Babayeva, S., Akparov, Z., Amirov, L., Shikhaliyeva, K., Hasanova, S., Rustamov, K., Mirzayev, R., Izzatullayeva, V., Mirzaliyeva, I., & Mammadov, A. (2018). Genetic relationship among introduced lentil germplasm using agronomic traits and ISSR markers. Genetika, 50(2), 575-590.

Bedard, T., Risula, D., Olekson, A., & Saskatchewan, T. (2010). Pulse growers overview of the canadian pulse industry 2009. Canada: Agriculture and Agri–Food Canada.

Beyene, Y., Botha, A.-M., & Myburg, A. A. (2005). A comparative study of molecular and morphological methods of describing genetic relationships in traditional Ethiopian highland maize. African journal of biotechnology, 4(7), 586-595.

Bornet, B., Muller, C., Paulus, F., & Branchard, M. (2002). Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using tri-and tetra-nucleotide primers from DNA of cauliflower (Brassica oleracea var. botrytis L.). Genome, 45(5), 890-896.

Coyne, C., & McGee, R. (2013). Lentil. In Genetic and Genomic Resources of Grain Legume Improvement (pp. 157-180). Elsevier.

de la Vega, M. P., & Vinagre, M. Y. D. (2004). Assessment of genetic variation and species relationships in a collection of" Lens" using RAPD and ISSR. Spanish journal of agricultural research(4), 538-544.

FAO. (2021). SPECIAL REPORT: 2021 Fao Crop and Food Supply Assessment Mission to the Syrian Arab Republic-December 2021. FAO.

Fikiru, E., Tesfaye, K., & Bekele, E. (2007). Genetic diversity and population structure of Ethiopian lentil (Lens culinaris Medikus) landraces as revealed by ISSR marker. African journal of biotechnology, 6(12).

Joshi, M., Verma, S., Singh, J., & Barh, A. (2013). Genetic diversity assessment in lentil (Lens culinaris Medikus) genotypes through ISSR marker. The Bioscan, 8(4), 1529-1532.

Kolodinska Brantestam, A., Von Bothmer, R., Dayteg, C., Rashal, I., Tuvesson, S., & Weibull, J. (2004). Inter simple sequence repeat analysis of genetic diversity and relationships in cultivated barley of Nordic and Baltic origin. Hereditas, 141(2), 186-192.

Lassner, M. W., Peterson, P., & Yoder, J. I. (1989). Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant molecular biology reporter, 7, 116-128.

Mbasani-Mansi, J., Ennami, M., Briache, F. Z., Gaboun, F., Benbrahim, N., Triqui, Z. E. A., & Mentag, R. (2019). Characterization of genetic diversity and population structure of Moroccan lentil cultivars and landraces using molecular markers. Physiology and Molecular Biology of Plants, 25(4), 965-974.

Pandey, A. K., Sengar, R. S., Kumar, A., Chand, P., & Yadav, R. (2018). Molecular characterization of Lentil (Lens culinaris Medikus) genotypes through Simple sequence repeat (SSR) markers. Biotech Today: An International Journal of Biological Sciences, 8(1), 65-72.

Seyedimoradi, H., & Talebi, R. (2014). Detecting DNA polymorphism and genetic diversity in Lentil (Lens culinaris Medik.) germplasm: comparison of ISSR and DAMD marker. Physiology and Molecular Biology of Plants, 20(4), 495-500.

Toklu, F., Karaköy, T., Haklı, E., Bicer, T., Brandolini, A., Kilian, B., & Özkan, H. (2009). Genetic variation among lentil (Lens culinaris Medik) landraces from Southeast Turkey. Plant Breeding, 128(2), 178-186.

Xlstat, X. (2017). Data analysis and statistical solution for Microsoft Excel. Addinsoft Paris, France.

Zietkiewicz, E., Rafalski, A., & Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2), 176-183.

• PDF

Copyright (c) 2023 Rehab Al-Mousa, Alaa Alshaal, Shahinaz Abbas, Khouzama Kountar

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Copyright of the articles Published by Almukhtar Journal of Science (MJSc) is retained by the author(s), who grant MJSc a license to publish the article. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors and cite MJSc as the original publisher. Also, they accept the article remains published by the MJSc website (except in the occasion of a retraction of the article).