Effect of sucrose, pH and medium states on in vitro shoot formation and growth of Moris pineapple (Ananas comosus L. Merr.)

• Abdelhamid M. Hamad

  Horticulture Department, Faculty of Agriculture, Omar Al-Mukhtar University, Libya

  Author

The effect of medium states (solid, semi solid and liquid) of full strength MS medium enriched with  6-benzylaminopurine (BAP) at 2.0 mg/l on in vitro shoots formation and shoot length of Moris pineapple were tested at 16 combinations of sucrose (10, 20, 30 and 40 g/l) and pH (5.0, 5.7, 6.0 and 6.5). The highest shoot formations (7 shoots/ explant) were obtained in liquid and solid media each adjusted to pH 5.0 but enriched with different sucrose concentrations, sucrose at 20 g/l for liquid and at 30 g/l for solid medium. Increasing the medium sucrose to 40 g/l or adjusting the medium to pH 6.0 caused 50 % decline in the shoot formation capacity in both medium states. However, while that decline could be reversed in liquid medium by adjusting the pH to 6.5, such pH adjustment failed to overcome the inhibitory effect of the sucrose at 40 g/l in the solid medium. Out of 16 combinations of sucrose and pH, liquid medium (no agar added) was better than solid (7.0 grams of agar /l) and semi solid (3.5 grams of agar /l) at 8 combinations, equal to solid at 4 and to semi solid at 5 combinations and less than solid at 3 and than semi solid at 2 combinations. Adopting of the commonly used  combination of sucrose at 30 g/l and pH 5.7 not only did not fit all medium states but also resulted in lower shoot formation (4 shoots) than the possibly obtainable (7 shoots). Simple modification of the medium pH (pH 5.0 instead of 5.7) doubled the rate of shoot formation.

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