Amplified Fragment Length Polymorphism (AFLP)

• Khaled Elmeer

  Horticulture Department, Faculty of Agriculture, Omar AL-Mukhtar University Libya

  Author

• Mohamed Bakory

  Animal production Department Faculty of Agriculture, Sabha University Libya

  Author

Amplified Fragment Length Polymorphism (AFLP)is one of the most important molecular marker techniques which is used for fingerprinting and genetic diversity study, AFLP based on detecting of DNA fragments which are digested by restriction enzymes then amplified by polymerase chain reaction PCR, the fragments are visualized on polyacrylamide gels or by using fluorescent detection in capillary systems. This article provides a detailed review for principles, methodologies, applications, advantages and limitations of AFLP.

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