Inventory of Lactobacteria in the Legume Seeds and Separate their Toxins

• Nawara Ali Mohamed

  Department of Plant Protection, Faculty of Agriculture, Omar Al-Mukhtar University, Al-Bayda, Libya

  Author

• Nawara Ali Mohamed

  Department of Plant Protection, Faculty of Agriculture, Omar Al-Mukhtar University, Al-Bayda, Libya

  Author

• Najwa Abdel Sattar Ibrahim

  Department of Plant Protection, Faculty of Agriculture, Omar Al-Mukhtar University, Al-Bayda, Libya

  Author

The aim of this study was to isolate the fungi borne on the seeds of some legumes and to extract their toxins. Six types of dried legume seeds were collected from the local market (Benghazi to Tobrok) in the such as: white bean, chickpea, faba beans, pea, black eyed pea, lentil, The results showed that there were differences among the areas of study and that there were significant differences between the seeds. The highest presences of fungi was found on the white bean especially in Benghazi and Tobruk, where the percentage were 92% and 65%, respectively. The chickpea and pea did not exceed 30%, while faba bean recorded 25% and 27.5% in Benghazi and Tobruk, respectively. These results also showed that the lentil seeds were free of fungi.the seeds were carried fungi such as: Penicillium sp, Botrytis, Fusarium sp, Sclerotinia, Asperigullus flvus, Rhizopus sp, Alternaria sp. Asperigullus niger, Asperigullus sp, these fungi were isolated after incubation in dishes contained Agar medium at 25±5°C. The presence of aflatoxins was the highest in the seeds of chick pea followed by bean and faba bean seeds in Benghazi and Tobruk, respectively, no toxins were detected in pea, black eyed pea, and lentil seeds.

أبوغرسة، صلاح الدين مصطفى (2001) أساسيات علم وتقنية البذور. ،منشورات جامعة عمر المختار، دار الكتب الوطنية - بنغازي، ليبيا.

الجالي، زهرة إبراهيم (1996) "تلوث بذور بعض المحاصيل بسموم الافلاتوكسينات بالجبل الأخضر. رسالة ماجستير، جامعة عمر المختار، البيضاء، ليبيا.

العيد، دهيمات، بوزيان، زهيره، نورالدين، قاسم شاوش، عبدالحكيم، بلعيدى وعبدالعزيز، وداد (2009) تأثير السموم الفطرية لعزلة الفطرAspergillus fumigatus فى انبات بذور بعض أصناف الحمص. مجلة جامعة دمشق للعلوم الزراعية، 25، 95-106.

بو حليقة، الطاهر أحمد(2009) حصر للفطور المرفقة للحبوب، مجلة وقاية النبات العربي ، ملخصات المؤتمر العاشر لوقاية النبات، 27، 50.

سرحان، عبد الرضا طه (2009) تشخيص الفطور المحمولة بالبذور والمرافقة لبذور بعض البقوليات وأسلوب مكافحتها حيويا في العراق. مجلة وقاية النبات العربية، 27، 135- 144.

عبدالرحيم، عوض محمد والعوشار، محمد عبدالجواد (1995) أمراض البذور. منشورات جامعة عمر المختار، دار الكتب الوطنية - بنغازي، ليبيا.

عبدالله، نجاح سليمان (2006) عزل واختبار القدرة الإمراضية للفطريات الممرضة المحمولة على بذور الحمص بالجبل الأخضر، مجلة المختار للعلوم، 13، 93-101.

عبد فاتن نوري ملا ورمضان، نديم احمد (2010) الفطريات المنتجة لأنزيم السليوليز والمحمولة ببذور بعض أنواع البقوليات. مجلة علوم الرافدين، 21، 65-57.

ميخائيل، سمير وتركي، بيدر (1982) أمراض البذور. جامعة الموصل، وزارة التعليم العالي، العراق.

Abd Al-Hafez, A. (1988) Mycoflora of broad bean chickpea and Ientil seed in Egypt. Crypto gamie-mycologia 9, 335-343.

Agarwal, V.K. and Sinclair, J.B. (1987) Mechanism of seed infection. in Principles of seed pathology, CRC Press, Inc. 176.

Agarwal, V.K. and Sinclair, J.B. (1987) Seed transmition and inoculation. in Principles of seed pathology, CRC Press, Inc. 176.

Al-Abdalall, A.H.A. (2010) Pathogenicity of fungi associated with leguminous seeds in the Eastern kingdom of Saudi Arabia. African Journal of Agricultural Research, 5, 1117-1126.

Alexopoulos, C.J. and Mime, C.W. (1979) Interoduction Mycology. John Wiley and sons, 3rd Edition.

Marai, I.F., Asker, A.A. (2008) Aflatoxins in rabbit production: hazards and control. Tropical and subtropical Agroecosystems, 8, 1-28.

Barnett, H.L. and Hunter, B.B. (1998) Illustrated genera of imperfect fungi. The American Phytopathological Societ, 130- 132.

CMI (1966a) Description of Pathogenic fungi and bacteria. Commonwealth Mycological Institute. Kew, Surrey, England. No. 96. June,1966.

CMI (1966b) Description of Pathogenic fungi and bacteria. Commonwealth Agricultural Bureaux, No. 91. June,1966.

CMI (1966c) Description of Pathogenic fungi and bacteria. Commonwealth Agricultural Bureaux, No. 94. June,1966.

Dhole, A.C.and Gurme, M.K. (2013) Utilization of Withania Somnfera (L.)Dunal To Control Seed Borne Fungus Alteraria Alternata (Fr.) Keissler. J. Chem. Bio. Phy. Sci., .3, 723-726.

El-Kady, I.A., El-maghraby, O.M. and Saber, S. (1986) Halophilic or halot-olerant fungi of four seeds from Egypt. Cryptogamie-mycologie, 7, 289-293.

Habish, H.A. (1972) Aflatoxin in haricotbean and other pulses. Exp. Agric., 8, 135-173.

Janardhan, A., Subramanyam, D., Praveen Kumar, A., Reddi Pradeep, M. and Narasimha, G. (2011) Aflatoxin Impacts on Germinating Seeds. Annals of Biological Research, 2, 180-188.

Jones, B.D. (1972) Method of aflatoxin analysis. Report of the Tropical products Institute, 24, 35-39.

Kocabas CN, Coşkun, T., Yurdakök, M., Haziroğlu, R. (2003) The effects of aflatoxin B1 on the development of kwashiorkor in mice. Hum Exp Toxicol. Mar, 22, (3), 155-158.

Margaret, E., Neeraja, P.V. and Rajeswari, B. (2013) Screening of Seed Borne Mycoflora of Cicer arietinum L. Int.J.Curr.Microbiol.App.Sci, 2, (8), 124-130.

Mathur, S.B. and Kongsdal, O. (2003) Common laboratory seed health testing methods for detecting fungi. International Seed testing Association, Bassersdorf, CH- Switzerland, 425.

Sarhan, A.R.T. (2006) Identification of seed borne fungi associated with some leguminous seeds in iraq and their control, Ninth Arab Congress of Plant Protection, 19-23 November 2006, Damascus, Syria, 68-69.

Sharif, F.M., Sarhan, A.R., Hegazi, A.M., Fatah, A.H., and Salam, M. (1987) Fungi associated with broad bean seed and their control. Journal of Univ. Kuwait (Sci.), 14, 331-336.

Toussoun, T.A. and Nelson, P.E. (1976) Fusarium a Pictorial guide to the identitfication of Fusarium species according to the taxonomic system of snyder and Hansen. The pennsylvania state university press university park and London, Second Edition, 43.

Uegaki, R., Tsukiboshi, T. and Cai, Y. (2010) Aspergillus flavus producing aflatoxins isolated from materials of commercial feed in Japan. JARQ , 44, 421-427.

• PDF (Arabic)

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Copyright of the articles Published by Almukhtar Journal of Science (MJSc) is retained by the author(s), who grant MJSc a license to publish the article. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors and cite MJSc as the original publisher. Also, they accept the article remains published by the MJSc website (except in the occasion of a retraction of the article).