Bacteriological  and Antibiotic Resistance Patterns of Uropathogens: A Laboratory-Based Study for outpatients clinic  in Ajdabiya City Libya  [2025]

• Husam Dakheel

  Department of Biology, Faculty of Science, University of Ajdabiya, Libya

  Author

This study aimed to assess the prevalence of bacteria causing urinary tract infections (UTIs) and to identify common patterns of bacterial isolates along with their relationship to urine pH in patients visiting the Elite Clinic laboratory in Ajdabiya. A total of 60 urine samples were collected under sterile conditions from individuals suspected of having UTIs. Bacterial cultures were conducted using standard media, and the bacterial species were identified based on their growth characteristics and biochemical tests. Additionally, urine pH was measured during the analysis. The findings revealed that 48 samples (80%) showed positive bacterial growth, indicating a high incidence of UTIs. Gram-negative bacteria accounted for most isolates (70–75%), while Gram-positive bacteria represented a smaller proportion (25–30%). Among the identified pathogens, Escherichia coli was the most prevalent (40–45%), followed by Klebsiella spp. (15–20%) and other species at lower frequencies. Furthermore, most positive samples were associated with acidic urine conditions, with 60–65% of elevated bacterial growth observed at pH levels below 7, compared to 35–40% in alkaline urine. The study highlights a significant prevalence of UTIs in the area under investigation, with a predominance of Gram-negative bacteria, particularly E. coli. It underscores the influence of urine pH on bacterial growth and distribution, offering valuable insights into the dynamics of urinary pathogens.

Adjei, S. K., & Adjei, P. (2025). Antimicrobial Susceptibility Patterns of Uropathogens: A Retro-spective Study at a Primary Care Hospital in Ghana. Health Science Reports, 8(7), e71029.‏

Ahmed, E., Hussain, M. S., Sheikh, S. I., & Ejaz, M. (2014). Urinary tract bacterial pathogens and their sensitivity pattern. Journal of Rawalpindi Medical College, 18(2).‏

Aleinein, I. A., & Sokhn, E. S. (2024). Knowledge and prevalence of urinary tract infection among pregnant women in Lebanon. Heliyon, 10(17).‏

Alhazmi, A. H., Alameer, K. M., Abuageelah, B. M., Alharbi, R. H., Mobarki, M., Musawi, S., & Dhayhi, N. (2023). Epidemiology and antimicrobial resistance patterns of urinary tract in-fections: a cross-sectional study from Southwestern Saudi Arabia. Medicina, 59(8), 1411.‏

Baimakhanova, B., Sadanov, A., Trenozhnikova, L., Balgimbaeva, A., Baimakhanova, G., Orasym-bet, S., & Turgumbayeva, A. (2025). Understanding the Burden and Management of Uri-nary Tract Infections in Women. Diseases, 13(2), 59.‏

Brandstrom, P., & Hansson, S. (2022). Urinary tract infection in children. Pediatric Clinics, 69(6), 1099-1114.‏

Bunduki, G. K., Heinz, E., Phiri, V. S., Noah, P., Feasey, N., & Musaya, J. (2021). Virulence factors and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from urinary tract infections: a systematic review and meta-analysis. BMC infectious diseases, 21(1), 753.‏

El-Arifi, S., AbdAlla, E., Mahgoub, E. S., Fadol, B., & Elmubarak, R. (2024). Characteristics and antibiotic resistance patterns of urinary tract isolates in hospitalized and non-hospitalized pa-tients: a cross-sectional study in Khartoum, Sudan. BMC Infectious Diseases, 24(1), 1356.‏

Erinmez, M., & Ozturk, M. (2026). Urinary Tract Infection in Patients with Urolithiasis: A Large Retrospective Observational Study of Clinical Features and Microbiological Spectrum. Pathogens, 15(1), 98.‏

Farag, P. F., Albulushi, H. O., Eskembaji, M. H., Habash, M. F., Malki, M. S., Albadrani, M. S., & Hanafy, A. M. (2024). Prevalence and antibiotic resistance profile of UTI-causing uropatho-genic bacteria in diabetics and non-diabetics at the Maternity and Children Hospital in Jed-dah, Saudi Arabia. Frontiers in Microbiology, 15, 1507505.‏

Gao, J., Wu, F., Zhang, Y., Wu, J., Li, W., Su, N., & Wang, Y. N. (2026). Drug Resistance Patterns and Pathogen Distribution in Patients with Urinary Tract Infection in Suzhou, China: A Five-Year Retrospective Study (2020–2024). Infection and Drug Resistance, 1-15.‏

Gonzalez, M. J., Robino, L., Zunino, P., & Scavone, P. (2024). Urinary tract infection: is it time for a new approach considering a gender perspective and new microbial advances? Frontiers in Urology, 4, 1487858.‏

He, Y., Zhao, J., Wang, L., Han, C., Yan, R., Zhu, P., & He, W. (2025). Epidemiological trends and predictions of urinary tract infections in the global burden of disease study 2021. Scientific Reports, 15(1), 4702.‏

Herrera-Espejo, S., Domínguez-Miranda, J. L., Rodríguez-Mogollo, J. I., Pachón, J., Cordero, E., & Pachón-Ibáñez, M. E. (2024). Effects of pH on the Pathogenicity of Escherichia coli and Klebsiella pneumoniae on the Kidney: In Vitro and In Vivo Studies. International Journal of Molecular Sciences, 25(14), 7925.‏

Jalil, M. B., & Al Atbee, M. Y. N. (2022). The prevalence of multiple drug resistance Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infections. Journal of clinical laboratory analysis, 36(9), e24619.‏

Kaur, R., & Kaur, R. (2021). Symptoms, risk factors, diagnosis and treatment of urinary tract infec-tions. Postgraduate medical journal, 97(1154), 803-812.‏

Khan, M., Hussain, F., Naseem, M., Clementina, R., Gul, N., Habib, A., & Khan, I. (2024). Investi-gating Urinary Pathogens and Their Antibiotic Resistance: A Cross-Sectional Urine Culture Study. Cureus, 16(7).‏

Kundu, N., Mou, F. A., Samia, F. N., Urmi, C. Y., Hossain, M. S., & Ahmad, T. (2025). Knowledge-level, attitudinal-trend and preventive-practices on urinary tract infections among reproductive-aged female university students. Discover Public Health, 22(1), 587.‏

Larsson, D. J., & Flach, C. F. (2022). Antibiotic resistance in the environment. Nature Reviews Mi-crobiology, 20(5), 257-269.‏

Li, X., Fan, H., Zi, H., Hu, H., Li, B., Huang, J., & Zeng, X. (2022). Global and regional burden of bacterial antimicrobial resistance in urinary tract infections in 2019. Journal of clinical med-icine, 11(10), 2817.‏

Mancuso, G., Midiri, A., Gerace, E., Marra, M., Zummo, S., & Biondo, C. (2023). Urinary tract in-fections: the current scenario and future prospects. Pathogens, 12(4), 623.‏‏

Moghnia, O. H., Al Otaibi, H. S., Al Haqqan, A. M., Sokhn, E. S., Pathan, S. S., Abdulaziz, N. E., & Al-Sweih, N. A. (2025). Prevalence of Urinary Tract Infections and Antibiotic Suscepti-bility Patterns of Uropathogens among Neonates in Maternity Hospital, Kuwait: A Six-Year Retrospective Study. Medical Principles and Practice, 34(3), 250-261.‏

Sabtu, N., Enoch, D. A., & Brown, N. M. (2015). Antibiotic resistance: what, why, where, when and how? British medical bulletin, 116(1), 105-113.‏

Salam, M. A., Al-Amin, M. Y., Salam, M. T., Pawar, J. S., Akhter, N., Rabaan, A. A., & Alqumber, M. A. (2023, July). Antimicrobial resistance: a growing serious threat for global public health. In Healthcare (Vol. 11, No. 13, p. 1946). MDPI.‏

Scherberich, J. E., Funfstuck, R., & Naber, K. G. (2021). Urinary tract infections in patients with renal insufficiency and dialysis–epidemiology, pathogenesis, clinical symptoms, diagnosis and treatment. GMS infectious diseases, 9, Doc07.‏

Sror, N., Bornstein, G., Golan, L., Rimbrot, D., Barlev, Y., Freund, O., & Zornitzki, L. (2025). Rate and Predictors of Urine Culture Positivity Following Single-Dose Antibiotic Administration: a Prospective Single-Center Study. Infectious Diseases and Therapy, 1-13.‏

Sung, K., Nawaz, M., Park, M., Chon, J., Khan, S. A., Alotaibi, K., & Khan, A. A. (2024). Com-prehensive genomic analysis of uropathogenic E. coli: virulence factors, antimicrobial re-sistance, and mobile genetic elements. Pathogens, 13(9), 794.‏Challenges and Prospects. Mi-croorganisms, 13(12), 2714.‏

Wang, B., Wang, Y., Liu, H., Yu, M., Wang, S., Liu, L., & Tan, H. (2025). Current Scientific Ad-vances in Vaccines Against UTIs:

Wireko, S., Ngmaakan, G., Dwomoh, D., Hansen, C., Nartey, S., & Senu, E. (2025). Regional Bur-den of Urinary Tract Infection, Its Aetiologies, and Antibiotic Resistance Patterns, 2020–2023: A Retrospective Study. Health Science Reports, 8(11), e71503.‏

Yang, X., Chen, H., Zheng, Y., Qu, S., Wang, H., & Yi, F. (2022). Disease burden and long-term trends of urinary tract infections: A worldwide report. Frontiers in public health, 10, 888205.‏

Zhou, Y., Zhou, Z., Zheng, L., Gong, Z., Li, Y., Jin, Y., & Chi, M. (2023). Urinary tract infections caused by uropathogenic Escherichia coli: mechanisms of infection and treatment options. International journal of molecular sciences, 24(13), 10537.‏

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