Mycoflora Detection in Some Dry Plants Used in Libyan Folk Medicine

Zahra I. El-Gali; Mohamed A. Elghli

doi:10.54172/vrt21p67

Authors

Zahra I. El-Gali Department of Plant Protec-tion, Faculty of Agriculture, Omer Al-Mukhtar Universi-ty, Libya Author
Mohamed A. Elghli Department of Plant Protec-tion, Faculty of Agriculture, Omer Al-Mukhtar Universi-ty, Libya. Author

DOI:

https://doi.org/10.54172/vrt21p67

Keywords:

Medicinal Plant, Moisture content, Fungi, Mycotoxins, Libya

Abstract

laurel Bay, Guava and Senna were purchased from different herbalist shops in Al-Beyda city. Moisture content was determined, and the levels of fungal contamination were detected in all samples by incubating them on 2% water agar petri dishes and purifying on potato sucrose agar at 25 ± 1^oC. The higher moisture contents of 50% and 25% were recorded in Guava and Greek laurel Bay respectively. Seven different genera and 12 species were isolated and identified as Alternaria alternata, Aspergillus flavus, A. carbonarius, A. niger, A. parasiticus, Cheatomium globosm, C. funicola, Cheatomium sp., Cladosporium cladosporides, Phoma sp., Pealosporium herbarum and Ulocladium charturm. The higher diversity of 14 isolates and the higher frequency of 38.9% were found in guava following by 11 isolates and 30.6% in Greek laurel Bay.The relative density of contamination for fungi isolated revealed that A. alternata and A. niger are maximum (15.7%) followed by C. funicola (13.7%) and the minimum number of fungi recorded was 2% for P. herbarium. Screening tests for mycotoxins producibility in isolates of fungi by using vapor ammonia liquid on PSA medium, among which A. flavus, A. carbonarius, A. niger, showed positive results for mycotoxins production.

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