التمييز بين المواطن البيئية لنبات الميرسين Myrtus communis  من خلال الترحيل الكهربائي لبروتينات التخزين الخضري Vegetative Storage Protein Electrophoresis

خديجة الحداد; أبوعجيلة سعد  أبوعجيلة; خلود فرج  عبدالمولي

doi:10.54172/x2yjtk73

Authors

khadiga alhadead Department of Environmental Sciences, Faculty of Natural Resources and Environmental Sciences, Omar Al-Mukhtar University, Libya Author https://orcid.org/0000-0001-9765-8746
Abuajila Alzntany Department of Pastures, Faculty of Natural Resources and Environmental Sciences, Omar Al-Mukhtar University, Libya Author
kholoud Faraj Department of Environmental Sciences, Faculty of Natural Resources and Environmental Sciences, Omar Al-Mukhtar University, Libya Author

DOI:

https://doi.org/10.54172/x2yjtk73

Keywords:

Environmental habitat, Vegetative Storage Protein Electrophoresis, Myrtus communis

Abstract

The growing interest in using Myrtus communis in industrial, pharmaceutical, and chemical applications has put this plant at risk due to overharvesting. These Plants occur in small, scattered populations, and their numbers are gradually declining. There are currently no effective programs in place to protect, enhance, or sustainably manage this resource. This study involved collecting Myrtus communis samples from two sites in the Jabal Akhdar region (Wadi Kuf, a relatively humid valley, and Wadi Azlifat, a drier valley), where the plant grows naturally. SDS-PAGE analysis of the plant's storage proteins revealed Pronounced variability in the number of protein bands, the percentage of variation, and the molecular weight. Samples from Wadi Kuf showed fewer bands (12) but a higher percentage of variation (42.85%), suggesting considerable genetic diversity within the population despite the lower number of bands. Samples from Wadi Azlifat showed a higher number of bands (14) but a lower percentage of variation (33.33%), possibly indicating greater homogeneity or similarity in protein profiles among individuals. The differences in molecular weight suggest that the two populations possess diverse proteins in terms of size and type, demonstrating the effect of different environmental factors, such as humidity, soil type, temperature, and other environmental stresses, concerning the expression of storage protein genes in Myrtus communis.

References

Abdelaziz, Mint, S., Medraoui, L., Alami, M. (2020). Inter simple sequence repeat markers to assess genetic diversity of the desert date (Balanites aegyptiaca Del.) for Sahelian ecosystem restoration. Sci Rep 10, 14948 (2020). https://doi.org/10.1038/s41598-020-71835-9.

Alhadead KM, Almesmari NM. (2021). Ecological and ethnobotanical study of Myrtus com-munis L.(Myrtaceae) in Al-Jabal Al-Akhdar, Libya. Libyan: J Ecol & Environ Sci Technol. (LJEEST) 2021;3(2):10–18. https://doi.org/10.5281/zenodo.4567890

Ali, K., Ullah, F., Khan, N., Rahman, I. U., Ullah, S., Khan, W. and Nisar, M. (2017). Ethnobo-tanical and ecological study of Myrtus communis (L.) in Bajaur agency (FATA) Khyber-Pakhtunkhwa, Pakistan. J of Biod and Envir Sci (JBES), 11(1), 152-164.

Amel B. 2013. Traditional treatment of high blood pressure and diabetes in

Souk Ahras District. J. Pharmacognosy Phytother 2013; 5(1): 12-20.https://doi: 10.5897/JPP11.065

Atoyebi, O. J.; Faluyi, J.O. and Oyedapom, O.O. (2014). Investigation of the genetic diversity of selected wild and cultivated Sorghum germplasm using sodium dodecyl sulphate polyacrylamide gel electrophoresis. Greener Journal of Biological Sciences 4: 001-008

Dukic, M.N.; D. Bugarin; S. Grbovic; C.D. Mitic; G.B. Vukovic; D. Orcic; E. Jovin; and M. Couladis; (2010). Essential oil of Myrtus communis L. as a potential antioxidant and antimu-tagenic agent molecule. 15: 2759- 2770. https://doi:10.3390/molecules15042759.

Emre, I. (2011). Determination of genetic diversity in the Vicia L. (Section Vicia) by using SDS-PAGE. Pakistan Journal of Botany 43: 1429-1432

Laemmeli U. K., (1970). Clevage of structural proteins during the assembly of the head of bacteri-ophage T4. Nature, 227, pp : 680-685. https://doi: 10.1038/227680a0

Mareri, L., Parrotta, L., & Cai, G. (2022). Environmental Stress and Plants. International Journal of Molecular Sciences, 23(10), 5416. https://doi.org/10.3390/ijms23105416

Mimica Dukic N, Bugarin D, Slavenko, Grbovic S, MiticCulafic D, VukovicGacic B, Orcic D, Jovin E, Couladis M. (2010). Essential Oil of Myrtus communis L. as a potential antioxi-dant andanti-mutagenic agents. Journal of Molecules 15, 2759-2770. https://doi.org/10.3390/molecules15042759

Mir, M. A. (2023). Myrtus Communis Leaves; Source of Bioactives, Traditional Use and Their Bio-logical Properties. https://doi.org/10.20944/preprints202301.0532.v1

Mitra, S. K., Irenaeus, T. K. S., Gurung, M. R., & Pathak, P. K. (2012). Taxonomy and im-portance of Myrtaceae. 959, 23–34. https://doi.org/10.17660/ACTAHORTIC.2012.959.2

Mostafa, E., Chaachouay, N., Douria, A., Zidane, L., Ouasti, I., and Bussmann, Rainer. (2023). Myrtus communis L. Myrtaceae. 10.1007/978-3-031-13933-8_148-1. https://doi.org/10.1007/978-3-031-13933-8_148-1

Mulas M, Cani MR (1999). Germplasm evaluation of spontaneous myrtle (Myrtus communis L.) for cultivar selection and crop development. J Herbs Spices Med Plants 6: 31–49. https://doi.org/10.1300/J044v06n03_04

Mulas, M.; M.R. Cani; and N. Brigaglia (1998). Characters useful to cultivation in spontaneous populations of Myrtus communis L. Acta Horticult., 457:271–278. https://doi.org/10.17660/ActaHortic.1998.457.34

Paterson, A.H.; Bowers, E.J.; Bruggmann, R.; Dubchak, I.; Grimwood, J.; Gundlach, H.; Haberer, G. and Hellsten, U. (2009). The Sorghum bicolor genome and diversification of grasses. Nature 457: 551-556. https://doi.org/10.1038/nature07723

Qari, S.H.; Kamel, E.A. and Sayed, H.E. (2016). Characterization and identification of seed stor-age protein of twelve lettuce cultivars. Bioscience Biotechnology Research Asia 13: 1959-1967. https://doi.org/10.13005/bbra/2350

Santos, J., Herrero, M., Mendiola, J. A., Oliva-Teles, M. T., Ibáñez, E., Delerue-Matos, C., and Oliveira, M. B. (2020). Nutritional and phytochemical profiles and biological activities of Myrtus communis L. berries. Journal of Food Composition and Analysis, 87, 103389. https://doi.org/10.1016/j.jfca.2020.103389

Singh S. P., Gepts , Debouck D. G. , (1991). Races of common bean (Phaseolus vulgaris. Fabace-ae). Econ. Bot. 45, pp : 379-396. https://doi.org/10.1007/BF02887079

The IUCN Red List of Threatened Species. https://www.iucnredlist.org

Yadegarinia, D., Gachkar, L., Rezaei, M. B., Taghizadeh, M., Astaneh, S. A., and Rasooli, I. (2006). Biochemical activities of Iranian Myrtus communis L. essential oil. Phytochemistry, 67(18), 1973–1978. https://doi.org/10.1016/j.phytochem.2006.06.005