Influence of Soil pH on Azotobacter Population With Using Microbiological Characteristics as Bio-Measurement in Arable Lands of Tripoli. N. W. Libya
- Authors
-
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Merfat T. Ben Mahmud
Department of Soil and Water, Faculty of Agriculture, Tripoli University, Tripoli -LibyaAuthor -
Eman A. Ferjani
Department of Soil and Water, Faculty of Agriculture, Tripoli University, Tripoli -LibyaAuthor
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- Keywords:
- Azotobacter, Soil properties, population
- Abstract
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In the present study, 15 samples of soil were collected to isolate Azotobacter from the rhizosphere in different regions of Tripoli. LG specified medium was used for the isolation of bacteria and were purified on the same medium for identification and characterisation.The colonies were identified through microscopical and biochemical tests and the results obtained were classified as Azotobacter sp. Subsequently, the microbial population was calculated by colony count method. The soil pH, total nitrogen content (N), total phosphorus content (P) and organic carbon (OC) in soil were determined. The results of this study indicated to effects positive and negative of soil pH levels on Azotobacter population. In the estimation of above chemical properties of all soil samples it was showed that bacterial population differs significantly among the different soil samples.
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- References
-
Ahmad, F. Ahmad, I. and Khan, M. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological Research 163(2):173-181.
Bashan, Y. (1990). Short exposure to Azospirillum brasilense Cd inoculation enhanced proton efflux of intact wheat roots. Canadian Journal of Microbiology 36(6):419-425.
Becking, J.-H. (1981). The family Azotobacteraceae. Pages 795-817 The prokaryotes. Springer.
Becking, J. H. (1992). The family Azotobacteraceae. Pages 3144-3170 The Prokaryotes. Springer.
Benizri, E. Baudoin, E. and Guckert, A. (2001). Root colonization by inoculated plant growth-promoting rhizobacteria. Biocontrol Science and Technology 11(5):557-574.
Campbell, C. Zentner, R. Selles, F. Biederbeck, V. McConkey, B. Blomert, B. and Jefferson, P. (2000). Quantifying short-term effects of crop rotations on soil organic carbon in southwestern Saskatchewan. Canadian Journal of Soil Science 80(1):193-202.
Channal, H. Alagawadi, A. Bharamagoudar, T. Udupa, S. Patil P. and Mannikeri, I. (1989). Azotobacter population as influenced by soil properties in some soils of North Karnataka. Current science. Bangalore 58(2):70-71.
Dart, P. and Day, J. (1975). Nitrogen fixation in the field other than by nodules. Soil Microbiology. Butter Worth Sci. Publication, London.
Dobereiner, J. and Pedrosa, F. O. (1987). Nitrogen-fixing bacteria in nonleguminous crop plants. Science Tech Publishers.
Gonzalez‐Lopez, J. Martinez‐Toledo, M. Reina, S. and Salmeron, V. (1991). Root exudates of maize and production of auxins, gibberellins, cytokinins, amino acids and vitamins by Azotobacter chroococcum in chemically‐defined media and dialysed‐soil media. Toxicological & Environmental Chemistry 33(1-2):69-78.
Hammad, A. (1998). Evaluation of alginate‐encapsulated Azotobacter chroococcum as a phage‐resistant and an effective inoculum. Journal of Basic Microbiology 38(1):9-16.
Jensen, V. and Petersen, E. (1955). Taxonomic studies on Azotobacter chroococcum Beij. and Azotobacter beijerinckii Lipman. Royal Vet. Agric. Coll. Copenhagen Yearbook:107-128.
Kanungo, P. Ramakrishnan, B. and Rao, V. R. (1997). Placement effects of organic sources on nitrogenase activity and nitrogen-fixing bacteria in flooded rice soils. Biology and Fertility of Soils 25(2):103-108.
Kennedy, C. Rudnick, P. MacDonald, M. and Melton, T. (2005) Genus III: Azotobacter. In: Garrity GM, editor. Bergey's Manual of Systematic Bacteriology. The Proteobacteria, Part B, the Gammaproteobacteria. 2nd edition. Vol. 2. New York, USA: Springer. 384- 402.
Kennedy, A. C. and Smith, K. (1995). Soil microbial diversity and the sustainability of agricultural soils. Plant and Soil 170(1):75-86.
Olsen, S. R. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate. United States Department Of Agriculture; Washington.
Pandey, R. Bahl, R. and Rao, P. (1986). Growth stimulating effects of nitrogen fixing bacteria (biofertiliser) on oak seedlings. Indian Forester 112(1):75-79.
Rousk, J. Bååth, E. Brookes, P. C. Lauber, C. L. Lozupone, C. Caporaso, J. G. Knight, R. and Fierer, N. (2010). Soil bacterial and fungal communities across a pH gradient in an arable soil. The ISME Journal 4(10):1340.
Shehata, S. Saleh, S. and Junge, H. (2005). Response of sexual expression and productivity of squash plants to some biofertilizer treatments. Egypt Journal Applied Science 20(12B):680-690.
Tejera, N. Lluch, C. Martinez-Toledo, M. and Gonzalez-Lopez, J. (2005). Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere. Plant and Soil 270(1):223-232.
Terzaghi, B. E. (1980). Ultraviolet sensitivity and mutagenesis of Azotobacter. Microbiology 118(1):271-273.
Vikhe, P. (2014). Azotobacter species as a natural plant hormone synthesizer. Research Journal of Recent Sciences 3:59-63.
Walky, A. and Black, I. (1934). An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid in soil analysis. 1. Experimental. Soil Sciences 79:459-465.
Wardle, D. (1992). A comparative assessment of factors which influence microbial biomass carbon and nitrogen levels in soil. Biological Reviews 67(3):321-358.
Wu, S. Cao, Z. Li, Z. Cheung, K. and Wong, M. (2005). Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma 125(1-2):155-166.
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- 2018-06-30
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- Vol. 33 No. 2 (2018)
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Copyright (c) 2021 Merfat T. Ben Mahmud, Eman A. Ferjani

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