Biological Control of Early Blight Disease on Tomato Plant on Al-Gabal Al-Akhdar District

• Nwara A. Mohamed

  Plant Protection Department, Omar Al-Mukhtar University, P.O. 919, Al-Bayda - Libya.

  Author

• Mohammed A. Saeed

  Plant Protection Department, Omar Al-Mukhtar University, P.O. 919, Al-Bayda - Libya.

  Author

• M.G. El-Samman

  Department of Agricultural Botany, Ain Shams University, Arab Republic of Egypt.

  Author

• Issa A. Abugharsa

  Plant Protection Department, Faculty of Agriculture, Omar Al-Mukhtar University, Al-Bayda-Libya

  Author

This study was conducted in Faculty of Agriculture Omar Al-Mukhtar University during the period 1999-2001 in which we tested the effects of microorganisms (Bacteria and fungi) that isolated from phylloplane of some Solanaceae and rhizosphere of some vegetable crop as biological agents to control Alternaria solani, 17 bacterial species belongs to (Flavobacterium, Micrococcus, Bacillus and Pseudomonas) and six Fungal genera belongs to (Penicillium, Alternaria, Aspergillus, Fusarium, Ulocladium and Stemphylium) were isolated from the phylloplane of some solanaceae, and 30 bacterial species from the genera (Bacillus and Pseudomonas) were isolated from the rhizosphere. The results of double antagonistic in the lab. Showed that Bacillus spp. Gave the highest antagonistic effect against A. solani followed by the Micrococcus and Pesudomonas species. On the other hand no inhibition zone were noticed when using fungal species except inhibition resulting from direct hyphal contact. The results of field studies indicated that the disease severity of early blight was also reduced when the leaves of tomato blants were inoculated with the causal agent of the disease and treated with Clavibacter sp. That isolated from phylloplane of pepper plants.

التهامي ، ر.أ. ؛ علي ، أ.ز. ؛ أبو زيد ، م.إ. ؛ عبد المعطي ، ت.ح. ؛ شلبي ، م.ص.

(1994) . المكافحة الكيماوية والحيوية لمرض عفن ثمار البندورة / الطماطم والفلفل التي تحدثها أنواع مختلفة من Alternaria . مجلة وقاية النبات العربية 43 : 12 .

Barnett. H.L.; and Hunterm B.B. (1972). Illustrated genera of imperfect fungi. 3rd Ed. Burgress publishing Co., Minnesota, U.S.A. 241 pp.

Fravel, D.R.; and Spurr, G. (1977). Biocontrol of tobacco brown-spot disease by Bacillus cereus sub sp. Mycoides in control environment. Phytopathology 67: 932.

Holt, J.G.; Krieg, N.R.; Staley, J.T.; and Williams, S.T. (1994). Bergey’s manual of determinative bacteriology, 9th Ed. Williams & Wilkins. Blatimore, U.S.A. 1347 pp.

Johanson, L.F.; Curl, E.A.; Bond, J.H.; and Fribourg, H.A. (1959). Method for studying soil microflora. Plant Disease Relationship. Minneapolis, Burgess Publication company. 366 pp.

Niwas, S.; and Sharma, P.D. (1988). Biological inhibition of Alternaria solani Current, Science. 57: 1243.

Okasha, A.M.; Sharif, F.M.; Al-Hussein, F.A. and Jemeel, W.N. (1989). Effect of some fungicides and antagonistic fungi on infection of tomato with leaf mold and early blight in plastic greenhouse. Arab. J. Plant Protection. 7: 126-132.

Reynard, G.B.; and Ardreus, C.F. (1945). Resistance to septoria leaf spot and its inheritance in tomatoes, Phytopathology 35: 16-24.

Vakalounakis, D.J. (1991). Control of early blight of greenhouse tomato, caused by Alternaria solani, by inhibiting sporulation with ultraviolet-absorbing vinylfilm. Plant Disease. 75: 795-797.

Whitesides, S.K.; and Spotts, R.A. (1991). Induction of pear blossom blast by Pseudomonas syringae pv. Syringae. Plant pathology 40: 118-127.

Xuechi, F.; Zhinong, Y.; Weimin, X.; Ruihong, M.; and Chai, C. (1997). Study on the biological control of Alternaria solani f. sp. mali and Botryosphaeria berengeriana of apple trees. China fruits 3: 7-10 (c.f. Rev. Plant Pathol. 77: 556. 1998)

Zaher, E.; Barakat, F.; Osman, A.; and El-khaleely, M. (1985). Antagonism between Phyllospere bacteria and actinomycetes and Locladium botrytis, causing tomato leaf spot. Egypt. J. Phytopathol 17: 15-22.

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