Comparative Study Using Simple Methods in Preservation of Some Microorganisms

• Wail Al–Dabbagh

  Department of Biology, Faculty of Science - Omar Al-Mukhtar University, Al-Bayda - Libya.

  Author

• Aala R. Ali

  Department of Biology, Faculty of Science - Omar Al-Mukhtar University, Al-Bayda - Libya.

  Author

Freezing (–20°C) in corn oil, agar disc, gelatin disc, filter paper and wheat seeds were compared for preservation of single cultures of Pseudomonas spp., Escherichia coli, Lactobacillus bulgaricus, Streptococcus thermophilus and Saccharomyces cerevisiae up to 50 weeks.

All the cultures under investigation were found to preserve successfully with these methods of preservation except Lactobacillus bulgaricus which failed to maintain their viability with agar disc, gelatin disc and filter paper.

According to this study, it is suggested that using filter paper is of beneficial value in preserving Saccharomyces cerevisiae.

With Pseudomonas spp., Escherichia coli, and Streptococcus thermophilus

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