Bacteriological and Antibiotic Resistance Patterns of Uropathogens: A Laboratory-Based Study for outpatients clinic  in Ajdabiya City Libya [2025]

• Husam Dakheel

  Department of Biology, Faculty of Science, University of Ajdabiya, Libya

  Author

This study aimed to assess the prevalence of bacteria causing urinary tract infections (UTIs) and to identify common patterns of bacterial isolates along with their relationship to urine pH in patients visiting the Elite Clinic laboratory in Ajdabiya. A total of 60 urine samples were collected under sterile conditions from individuals suspected of having UTIs. Bacterial cultures were conducted using standard media, and the bacterial species were identified based on their growth characteristics and biochemical tests. Additionally, urine pH was measured during the analysis. The findings revealed that 48 samples (80%) showed positive bacterial growth, indicating a high incidence of UTIs. Gram-negative bacteria accounted for most isolates (70–75%), while Gram-positive bacteria represented a smaller proportion (25–30%). Among the identified pathogens, Escherichia coli was the most prevalent (40–45%), followed by Klebsiella spp. (15–20%) and other species at lower frequencies. Furthermore, most positive samples were associated with acidic urine conditions, with 60–65% of elevated bacterial growth observed at pH levels below 7, compared to 35–40% in alkaline urine. The study highlights a significant prevalence of UTIs in the area under investigation, with a predominance of Gram-negative bacteria, particularly E. coli. It underscores the influence of urine pH on bacterial growth and distribution, offering valuable insights into the dynamics of urinary pathogens.

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