Bacterial Pathogens in Diabetic Foot Infections in Diabetes Center of Al-Bayda: Phenotypic Identification Using the Render MA 120 System
DOI:
https://doi.org/10.54172/48rnqy36Keywords:
Diabetic Foot, Libya, Klebsiella Pneumoniae, Gram-Negative Bacteria, Render MA 120, Opportunistic PathogensAbstract
Diabetic foot infection (DFI) is a major clinical complication of diabetes mellitus, serving as a primary precursor to lower-limb amputation. This study aimed to identify and phenotypically characterize the bacterial pathogens associated with DFIs at the Diabetes Center in Al-Bayda, Libya. Samples were collected from infected wounds, tissues, and nails of 14 patients and analyzed using the Render MA 120 automated identification system. Results showed a high prevalence of Gram-negative bacteria (90%), with Klebsiella pneumoniae was the most frequent isolate (25.5%), followed by Escherichia coli (17.65%). Notably, rare opportunistic pathogens, including Burkholderia pseudomallei (1.96%) and Raoultella ornithinolytica (1.96%), were identified. These findings highlight a significant regional shift toward Gram-negative dominance and underscore the necessity for precise diagnostic tools and targeted empirical antibiotic protocols to prevent limb loss.
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