Molecular Characterization of Cucumber mosaic virus (CMV) local isolates in Libya
DOI:
https://doi.org/10.54172/1kg2b798Keywords:
Cucumber mosaic virus (CMV), Cucumber, Tomato, RT-PCR, Phylogenetic analysisAbstract
Abstract
Field observation revealed that the most common symptoms on naturally infected plants in Al-Marg City- Libya were severe mosaic, mild mosaic on Cucumber, pepper, tomato and eggplant. These symptoms were suspected of being caused by Cucumber Mosaic Virus (CMV) and confirmed by The Reverse transcription-polymerase Chain Reaction (RT-PCR). The test isolates demonstrated RT-PCR amplification product of 540 bp, Pure RT-PCR product of 2 Libyan CMV isolates (3TR_Tomato, 5CF_cucumber) were sent for sequencing. Comparison of coat protein sequences of two Libyan isolates from tomato and cucumber plant to that of other reported isolates in genbank, Both sequences received accession numbers of OM000303 and OM000304 from Genbank, The phylogenetic tree generated from alignment based on the nucleotide sequence comparison of the CMV coat protein of the 2 Libyan isolates CMV isolates and the 13 CMV isolates from different geographical areas showed by MEGA X, revealed that our Libyan isolates (OM000303 and OM000304) are clustered together in the same branch but there was a higher genetic difference between two Libyan isolate in the same clade. Libyan isolates are out grouped to other isolate in the tree, this is mean that we may got new CMV isolates
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