Anti-MRSA Activity using Brown algae (Padina pavonica & Sargassum vulgare), in vitro
DOI:
https://doi.org/10.54172/8jfw7856Keywords:
MRSA, Padina pavonica & Sargassum vulgare, Antibacterial.Abstract
Methicillin-Resistant Staphylococcus aureus (MRSA) is one of the most typical bacteria Infectious in Libyan hospitals. Therefore, the present study was conducted as an attempt to find an alternative treatment to reduce the infection MRSA, from crude acetone and ethanol extracts of brown algae (Padina pavonica and Sargassum vulgare) against three MRSA isolates: Wound (W), Skin (S) and diabetic foot ulcers (DFU), by comparing with three an antibiotics (Imipenem, Gentamicin and Tetracycline), the drug susceptibility test was performed using a disc diffusion technique. The obtained results showed that all extracts of brown algae showed a clear inhibition effect toward the growth of two isolates of MRSA (W) and (S). On the other hand, P. pavonica extracts are more efficient than S. vulgare extracts, especially acetone, MARSA (DFU) was the most resistant isolates to antibiotics and extracts.Imipenem showed good activity against a tested MRSA species, which is a noteworthy results. Generally, it can be concluded that acetone extracts of P. panovica may be a good source of compounds effective for anti-MRSA
References
S. Bhattacharya, K. Pal, S. Jain, S. S. Chatterjee, J. Konar, Surgical site infection by methicillin resistant staphylococcus aureus–On decline? Journal of clinical and diagnostic research: JCDR 10, DC32 (2016).
2. O. Schmalz, T. Strapatsas, C. Alefelder, S. O. Grebe, Methicillin-resistant Staphylococcus aureus in palliative care: A prospective study of Methicillin-resistant Staphylococcus aureus prevalence in a hospital-based palliative care unit. Palliative medicine 30, 703 (2016).
3. B. Tarai, P. Das, D. Kumar, Recurrent challenges for clinicians: emergence of methicillin-resistant Staphylococcus aureus, vancomycin resistance, and current treatment options. Journal of laboratory physicians 5, 071 (2013).
4. A. S. Viana, A. M. N. Botelho, A. M. Moustafa, C. L. Boge, A. L. P. Ferreira, M. C. da Silva Carvalho, P. J. Planet, Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil. Emerging infectious diseases 27, 2825 (2021).
5. M. Barber, Methicillin-resistant staphylococci. Journal of clinical pathology 14, 385 (1961).
6. K. M. Craft, J. M. Nguyen, L. J. Berg, S. D. Townsend, Methicillin-resistant Staphylococcus aureus (MRSA): antibiotic-resistance and the biofilm phenotype. MedChemComm 10, 1231 (2019).
7. N. Buzaid, A.-N. Elzouki, I. Taher, K. S. Ghenghesh, Methicillin-resistant Staphylococcus aureus (MRSA) in a tertiary surgical and trauma hospital in Benghazi, Libya. The Journal of Infection in Developing Countries 5, 723 (2011).
8. G. Alshaibani, A. Shahlol, A. Abid, S. Amri, E. Aghila, G. Abdalah, M. Ahmed, Characterization and antibiotic resistance of staphylococci strains isolated from brack hospital in the Southern Region of Libya. Libyan International Medical University Journal 6, 75 (2021).
9. I. H. Kim, J.-H. Lee, Antimicrobial activities against methicillin-resistant Staphylococcus aureus from macroalgae. Journal of Industrial and Engineering Chemistry 14, 568 (2008).
10. M. M. El-Sheekh, A. S. H. Mousa, A. A. Farghl, Antibacterial efficacy and phytochemical characterization of some marine brown algal extracts from the red sea, Egypt. Romanian biotechnological letters 25, 1160 (2020).
11. S. E. Shafay, M. El-Sheekh, E. Bases, R. El-Shenody, Antioxidant, antidiabetic, anti-inflammatory and anticancer potential of some seaweed extracts. Food Science and Technology 42, (2021).
12. D. González-Mendoza, B. Valdez-Salas, M. Carrillo-Beltran, S. Castro-Lopez, V. Méndez-Trujillo, F. Gutierrez-Miceli, N. Arce-Vazquez, Antimicrobial effects of silverphyconanoparticles from Sargassum vulgare against spoilage of fresh vegetables caused by Bacillus cereus, Fusarium solani and Alternaria alternata. International Journal of Agriculture and Biology 20, 1230 (2018).
13. K. Sahayaraj, S. Rajesh, J. Rathi, Silver Nanoparticles Biosynthesis Using Marine Alga Padina Pavonica (Linn.) And Its Microbicidal Activity. Digest Journal of Nanomaterials & Biostructures (DJNB) 7, (2012).
14. P. J. L. Geraldino, L. M. Liao, S.-M. Boo, Morphological study of the marine algal genus Padina (Dictyotales, Phaeophyceae) from southern Philippines: 3 species new to Philippines. Algae 20, 99 (2005).
15. T. Yoshida, Taxonomy of Sargassum. Korean J Phycol 4, 107 (1989).
16. C. Chiao-Wei, H. Siew-Ling, W. Ching-Lee, Antibacterial activity of Sargassum polycystum C. Agardh and Padina australis Hauck (phaeophyceae). African Journal of Biotechnology 10, 14125 (2011).
17. A. J. Driscoll, N. Bhat, R. A. Karron, K. L. O’Brien, D. R. Murdoch, Disk diffusion bioassays for the detection of antibiotic activity in body fluids: applications for the pneumonia etiology research for child health project. Clinical Infectious Diseases 54, S159 (2012).
18. H. F. Chambers, The changing epidemiology of Staphylococcus aureus? Emerging infectious diseases 7, 178 (2001).
19. S.-H. Eom, J. H. Park, D. U. Yu, J. I. Choi, J. D. Choi, M. S. Lee, Y. M. Kim, Antimicrobial activity of brown alga Eisenia bicyclis against methicillin-resistant Staphylococcus aureus. Fisheries and aquatic sciences 14, 251 (2011).
20. N. Khaled, M. Hiba, C. Asma, Antioxidant and antifungal activities of Padina pavonica and Sargassum vulgare from the Lebanese Mediterranean Coast. Adv. Environ. Biol 6, 42 (2012).
21. K. Yu, S. Norhisham, N. CH, Antimicrobial potential of Padina australis and Sargassum polycystum against respiratory infections causing bacteria. International Journal of Medical Toxicology & Legal Medicine 22, 138 (2019).
22. M. Stańkowska, K. Garbacz, A. Korzon-Burakowska, M. Bronk, M. Skotarczak, A. Szyma´nska-Dubowik, Microbiological, Clinical and Radiological Aspects of Diabetic Foot Ulcers Infected with Methicillin-Resistant and-Sensitive Staphylococcus aureus. Pathogens 11, 701 (2022).
23. S. M. Palmer, M. J. Rybak, An evaluation of the bactericidal activity of ampicillin/sulbactam, piperacillin/tazobactam, imipenem or nafcillin alone and in combination with vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) in time-kill curves with infected fibrin clots. The Journal of antimicrobial chemotherapy 39, 515 (1997).
24. S. Gibbons, E. Udo, The effect of reserpine, a modulator of multidrug efflux pumps, on the in vitro activity of tetracycline against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) possessing the tet (K) determinant. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 14, 139 (2000).
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright of the articles Published by Al-Mukhtar Journal of Basic Sciences (MJBS) is retained by the author(s), who grant MJBS a license to publish the article. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors and cite MJBS as the original publisher. Also, they accept the article remains published by the MJBS website (except in the occasion of a retraction of the article). All MJBS Published articles under the Attribution-NonCommercial 4.0 International License.



