Effect of chitosan on proteomics in Lens culinaris Medik plants grown under different levels of salinity in vitro.
DOI:
https://doi.org/10.54172/vqpf6h14Keywords:
Chitosan, Culinaris, Salinity stress, Monomorphi, Polymorphism, ProteomicsAbstract
The present study aimed to investigate molecular responses of Lens culinaris Medik. (L. culinaris) plantlets to salinity stress concomitant with investigating the role of exogenous bio-stimulants application strategy chitosan (CTS) in ameliorating the deleterious effect of salt stress. In vitro-produced plantlets of L. culinaris were treated with varying levels of NaCl (50, 100 and 150 mM) added to MS solid media and/or a long with CTS (0.1 mg L-1). The molecular level, sodium dodecyl sulfate polyacrylamide gel electrophoresis technique (SDS-PAGE) for protein profile, was done. Analysing changes in SDS-PAGE protein banding patterns revealed a total number of 30 bands with molecular weights ranging from 15.2 KDa to 90.6 KDa. thirteen bands were observed as monomorphic, while seventeen bands were polymorphic, giving 43.33% polymorphism. Proteins banding was increased under salt stress compared to control induced as salt marker proteins.
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