Chemical composition of Ocimum sanctum by GC-MS Analysis

Ahmed A. Mustafa; Mubarak S.  Hamad; Haifa A.  Awad; Hatil  El-kamali

doi:10.54172/tr3mwd31

Authors

Ahmed A. Mustafa Department of Botany and Microbiol-ogy, Faculty of Science, University of Gezira, Wad-madani, Sudan Author
Mubarak S. Hamad Department of Taxonomy and Phy-tochemistry, Medicinal, Aromatic and Tradition Medicine,Research Institute, National Center for Research, Khartoum, Sudan Author
Haifa A. Awad Department of Botany, Faculty of Science, Sudan University of Science and Technology, Khartoum, Sudan Author
Hatil El-kamali Department of Botany Faculty of Science and Technology , Omdurman Islamic University, Omdurman, Sudan Author

DOI:

https://doi.org/10.54172/tr3mwd31

Keywords:

Ocimum sanctum, GC

Abstract

The chemical composition of the n-hexane extract from the aerial parts of Ocimum sanctum., was investigated using gas chromatography-mass spectrometry (GC/MS), identifying 46 different compounds. Terpenoids were the most abundant, with monoterpenes representing 21.82% of the extract. The primary components identified were methyl eugenol (27.24%), squalene (11.84%) α-bergamotene (9.83%), linalool (8.42%), and fenchyl acetate (7.56%). These results indicate that O. sanctum could serve as a valuable source of food and medicinal agents.

References

Desai, K., Wei, H., & Lamartiniere, C. (1996). The preventive and therapeutic potential of the squalene-containing compound, Roidex, on tumor promotion and regression. Cancer letters, 101(1), 93-96.

EL-Kamali, H. H., & EL-Amir, M. Y. (2010). Antibacterial activity and phytochemical screening of ethanolic extracts obtained from selected Sudanese medicinal plants. Current Research Journal of Biological Sciences, 2(2), 143-146.

Hassanpouraghdam, M. B., Hassani, A., & Shalamzari, M. S. (2010). Menthone-and estragole-rich essential oil of cultivated Ocimum basilicum L. from Northwest Iran. Chemija, 21(1), 59-62.

Hosseinzadeh, S., Jafarikukhdan, A., Hosseini, A., & Armand, R. (2015). The application of medicinal plants in traditional and modern medicine: a review of Thymus vulgaris. International Journal of Clinical Medicine, 6(9), 635-642.

Jirovetz, L., Buchbauer, G., Stoyanova, A., & Balinova, A. (2001). Analysis, chemotype and quality control of the essential oil of a new cultivated basil (Ocimum basilicum L.) plant from Bulgaria. Scientia Pharmaceutica, 69(1), 85-89.

Khair-ul-Bariyah, S. (2013). Comparison of the Physical Characteristics and GC/MS of the Essential Oils of Ocimum basilicum and Ocimum sanctum. International Journal of Scientific Research in Knowledge, 1(9), 363.

Kohno, Y., Egawa, Y., Itoh, S., Nagaoka, S.-i., Takahashi, M., & Mukai, K. (1995). Kinetic study of quenching reaction of singlet oxygen and scavenging reaction of free radical by squalene in n-butanol. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism, 1256(1), 52-56.

Mondello, L., Zappia, G., Cotroneo, A., Bonaccorsi, I., Chowdhury, J. U., Yusuf, M., & Dugo, G. (2002). Studies on the essential oil‐bearing plants of Bangladesh. Part VIII. Composition of some Ocimum oils O. basilicum L. var. purpurascens; O. sanctum L. green; O. sanctum L. purple; O. americanum L., citral type; O. americanum L., camphor type. Flavour and fragrance journal, 17(5), 335-340.

Mustafa, A. A., & El-kamali, H. H. (2019). Chemical composition of Ocimum americanum in Sudan. Res. Pharm. Health Sci, 5(3), 172-178.

Mustafa, A. A., & El-kamali, H. H. (2020). Proximate and phytochemical constituents of in Sudan Ocimum sanctum.

Nahak, G., Mishra, R., & Sahu, R. (2011). Taxonomic distribution, medicinal properties and drug development potentiality of Ocimum (Tulsi). Drug Invention Today, 3(6).

Omer, H. A., Mustafa, A. A., Kabbashi, A. S., & Taher, A. R. (2024). Antimicrobial, Antioxidant Activities and total phenolics contents of Portulaca oleracea., crude extracts, Sudan.

Park, I.-K., Kim, J., Lee, S.-G., & Shin, S.-C. (2007). Nematicidal activity of plant essential oils and components from ajowan (Trachyspermum ammi), allspice (Pimenta dioica) and litsea (Litsea cubeba) essential oils against pine wood nematode (Bursaphelenchus xylophilus). Journal of nematology, 39(3), 275.

Periyasamy Ashokkumar, P. A., Rajkumar, R., & Mahalingam Kanimozhi, M. K. (2010). Phytochemical screening and antimicrobial activity from five Indian medicinal plants against human pathogens.

Sabogal-Guáqueta, A. M., Osorio, E., & Cardona-Gómez, G. P. (2016). Linalool reverses neuropathological and behavioral impairments in old triple transgenic Alzheimer's mice. Neuropharmacology, 102, 111-120.

Sahreen, S., Khan, M. R., Khan, R. A., & Hadda, T. B. (2015). Evaluation of phytochemical content, antimicrobial, cytotoxic and antitumor activities of extract from Rumex hastatus D. Don roots. BMC complementary and alternative medicine, 15, 1-6.

Saint-Leger, S., Bague, A., Cohen, E., & Chivot, M. (1986). A possible role for squalene in the pathogenesis of acne. I. In vitro study of squalene oxidation. II. In vivo study of squalene oxides in skin surface and intra-comedonal lipids of acne patients.

Sfara, V., Zerba, E., & Alzogaray, R. A. (2009). Fumigant insecticidal activity and repellent effect of five essential oils and seven monoterpenes on first-instar nymphs of Rhodnius prolixus. Journal of medical entomology, 46(3), 511-515.

Yano, K. (1987). Minor components from growing buds of Artemisia capillaris that act as insect antifeedants. Journal of Agricultural and Food Chemistry, 35(6), 889-891.