The causes of Clinical and Subclinical Pathogenic Mastitis and anti-bacterial Resistance in Milk of Dairy Cows in and around Shahat, Libya

Ahmad Altalhy; Hamzah Othman

doi:10.54172/jqa2ce75

Authors

Ahmad Altalhy Department of microbiology and Immunology, Faculty of Dentistry, Tobruk University, Tobruk, Libya Author
Hamzah Othman 2 Department of pathology and Clinical pathology, Faculty of Veterinary Medicine, Omar AL.Mukhtar University, El beyda, Libya. Author

DOI:

https://doi.org/10.54172/jqa2ce75

Keywords:

antibacterial resistance, Shahat, Mastitis, Dairy Cows

Abstract

Overall, 200 milk samples were collected from January 2018 to March 2020, which led to the isolation of 126 pathogen agents (63%). Escherichia coli 46 (36.5%), Streptococcus pyogens 31 (24. 6%), and staphylococcus aureus 19 (15 %) were specified as the larger causative agents of clinical mastitis. While Streptococcus dysgalactiae 21 (16.7%) were mostly implicated in subclinical mastitis. With that, in both types of mastitis, also about 4 (3.2) % positive cases were caused by other bacterial agents that were identified at a low frequency (<4%) and cannot be considered as important pathogens like ( Klebsiella spp) or non-contagious like (Corynebacterium spp.). The antimicrobial resistance was moderate in the total proportion; exclude penicillin in staphylococci, and tetracycline in streptococci, Which are used as antimicrobial drug for humans’ treatment in Libya. In addition to collection the date information about mastitis issue in Libya.

References

Ahmed, W., Neubauer, H., Tomaso, H., El Hofy, F. I., Monecke, S., Abdeltawab, A. A., & Hotzel, H. J. P. (2020). Characterization of staphylococci and streptococci isolated from milk of bovides with mastitis in Egypt. 9(5), 381.

Awad, A., Ramadan, H., Nasr, S., Ateya, A., & Atwa, S. J. P. j. o. b. s. P. (2017). Genetic Characterization, Antimicrobial Resistance Patterns and Virulence Determinants of Staphylococcus aureus Isolated form Bovine Mastitis. 20(6), 298-305.

da Costa Krewer, C., Santos Amanso, E., Veneroni Gouveia, G., de Lima Souza, R., Da Costa, M. M., Aparecido Mota, R. J. T. a. h., & production. (2015). Resistance to antimicrobials and biofilm formation in Staphylococcus spp. isolated from bovine mastitis in the Northeast of Brazil. 47, 511-518.

Elsayed, M. S., & Dawoud, M. A. J. V. w. (2015). Phenotypic and genotypic detection of virulence factors of Staphylococcus aureus isolated from clinical and subclinical mastitis in cattle and water buffaloes from different farms of Sadat City in Egypt. 8(9), 1051.

Hamed, M., & Ziatoun, A. (2014). Prevalence of Staphylococcus aureus subclinical mastitis in dairy buffaloes farms at different lactation seasons at Assiut Governorate, Egypt. International Journal of Livestock Research, 4, 21. https://doi.org/10.5455/ijlr.20140526062755.

Hogan, J., Gonzalez, R., Harmon, R., Nickerson, S., Oliver, S., Pankey, J., & Smith, K. L. J. N. M. C., Madison, WI. (1999). Laboratory handbook on bovine mastitis. 78(7), 485-488.

Kaczorek, E., Małaczewska, J., Wójcik, R., Rękawek, W., & Siwicki, A. J. J. o. D. S. (2017). Phenotypic and genotypic antimicrobial susceptibility pattern of Streptococcus spp. isolated from cases of clinical mastitis in dairy cattle in Poland. 100(8), 6442-6453.

Li, D., Wang, Y., Schwarz, S., Cai, J., Fan, R., Li, J., . . . Shen, J. J. J. o. A. C. (2016). Co-location of the oxazolidinone resistance genes optrA and cfr on a multiresistance plasmid from Staphylococcus sciuri. 71(6), 1474-1478.

Oliver, S., & Calvinho, L. J. J. o. A. S. (1995). Influence of inflammation on mammary gland metabolism and milk composition. 73(suppl_2), 18-33.

Oliver, S. P., & National Mastitis, C. (2004). Microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality. (Fourth edition ed.). Verona, WI: NMC Verona, WI.

Qu, Y., Zhao, H., Nobrega, D. B., Cobo, E. R., Han, B., Zhao, Z., . . . Gao, J. J. J. o. d. s. (2019). Molecular epidemiology and distribution of antimicrobial resistance genes of Staphylococcus species isolated from Chinese dairy cows with clinical mastitis. 102(2), 1571-1583.

Reyher, K., Dufour, S., Barkema, H., Des Côteaux, L., Devries, T., Dohoo, I., . . . Scholl, D. J. J. o. d. s. (2011). The National Cohort of Dairy Farms—A data collection platform for mastitis research in Canada. 94(3), 1616-1626.

Ruegg, P. L. J. J. o. d. s. (2017). A 100-Year Review: Mastitis detection, management, and prevention. 100(12), 10381-10397.